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To create a new order, please fill out the form below.

* - Required fields

Contact Information
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*First Name:
*Last Name:
*Contact Email:
*Phone:
*Principal Investigator Email:
*Project/Task/Award (POETA) #:
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Protein Information
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*Protein Name:
*Molecular Weight:
1st Purification Tag:
2nd Purification Tag:
*Vector:
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Expression Information
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Cell Line:
Culture Conditions:
Expression Mode / Harvest:
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Additional Information
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Comments / Remarks:
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Order Information
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*Order Type:
Generation of baculovirus starting stock and expression optimization of recombinant protein expression
 Time required: 4 Weeks
 Cost: $600.00
Amplification up to 200ml of recombinant virus for high titer stock (5 ml virus stock required)
 Time required: 2 Weeks
 Cost: $100.00
 Quantity:
1L expression of target protein with the BEVS
 Time required: 1 Week
 Cost: $300.00
 Quantity:
1L expression of target protein with the BEVS with Selomethionin labeling
 Time required: 1 Week
 Cost: $600.00
 Quantity:
Expression Optimization verified by Western blot (5 ratios of infection, 2 time points, up to 2 cell lines)
 Time required: 1 Week
 Cost: $200.00
Baculovirus titer determination
 Time required: 2 Weeks
 Cost: $300.00
Plaque purification of recombinant virus
 Time required: 5 Weeks
 Cost: $400.00
30 ml transient (PEI) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $70.00
 Quantity:
30 ml transient (Lipofectamine) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $90.00
 Quantity:
100 ml transient (PEI) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $230.00
 Quantity:
100 ml transient (Lipofectamine) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $300.00
 Quantity:
1L transient (PEI) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $500.00
 Quantity:
1L transient (Lipofectamine) expression in mammalian HEK cells in suspension culture
 Time required: 1 Weeks
 Cost: $1200.00
 Quantity:
1L expression of target protein in Escherichia coli cells
 Time required: 1 Weeks
 Cost: $200.00
 Quantity:
Protein purification of 1L expression culture for target protein per purification step without concentrating step / column
 Time required: 1 Weeks
 Cost: $200.00
 Quantity:
Protein purification of 1L expression culture for target protein per purification step with concentrating step / column
 Time required: 1 Weeks
 Cost: $400.00
 Quantity:
Concentration of Protein from 1L to 200ml
 Time required: 0.5 Weeks
 Cost: $200.00
 Quantity:
Concentration and buffer exchange of Protein from 1L to 300ml
 Time required: 0.5 Weeks
 Cost: $300.00
 Quantity:
Transformation of plasmid DNA into E. coli expression host strain
 Time required: 1 Week
 Cost: $50.00
 
Custom / Other Item
Check this box to place a custom order. Please remember to include details about your order / item(s) in the Comments / Remarks section. We will contact you via phone or email once your order is received.
 Time required: To be determined
 Cost: To be determined
 
    
 
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Jost Vielmetter
Protein Expression Center
California Institute of Technology
Mail Code: 139-74
Pasadena, CA 91125